Kinetic Chromogenic Formulation
Chromo-LAL lysate is lyophilized with substrate reagent and buffers. It is optimized for the kinetic chromogenic LAL test method in microplate readers. Chromo-LAL is a buffered, stable and robust lysate, suitable for quantitative testing of a wide range of samples.
The sensitivity for chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Chromo-LAL is 0.005 EU/mL.
Sample to Lysate Ratio
In the Chromo-LAL test, reconstituted Chromo-LAL reagent is used at a ratio of 1:1 and a volume of 100 µL.
The Chromo-LAL/sample mixture is incubated at 37 ± 1°C in a microplate reader equipped with a 405 nm filter. Software is used to construct the standard curve and calculate the endotoxin concentrations.
The reconstituted lysate is stable for 24 hours at 2–8°C or for two weeks at -20°C or colder if frozen immediately after reconstitution. Reconstituted Chromo-LAL lysate may be frozen and thawed once.
Chromo-LAL lysate is sold by the vial for greater ordering and testing flexibility. This allows you to order the amount of lysate that is right for you.
|3.2 mL/Vial 5 Pack (150 tests)|