Introduction to the LAL Test
Limulus Amebocyte Lysate (LAL) tests detects and quantifies bacterial endotoxins extracted from the outer membrane of Gram-negative bacteria. The critical component of the LAL reagents used in endotoxin tests is derived from blood cells (amebocytes) of the horseshoe crab, Limulus polyphemus. It contains the proteins of the blood clotting mechanism, which is triggered by endotoxins. LAL reagents are primarily used to test for endotoxins in injectable pharmaceuticals, biological products, and medical devices. They are also used in renal dialysis centers and a wide range of other applications.
LAL tests are described in the Bacterial Endotoxins Test chapter in the United States Pharmacopeia and in the equivalent chapters in the European Pharmacopoeia and the Japanese Pharmacopoeia.

LAL Test Methods
There are three principal LAL test methods:

Extra
If you want to find more information regarding a particular subject visit our educational section. For tutorials on the kinetic turbidimetric or kinetic chromogenic method visit our YouTube channel.