Clotting based quantitative determination of Protein C


  • Activity assay
  • Very good on board stability (can be frozen)
  • Applications available for all analysers


The HEMOCLOT Protein C method is an APTT like clotting assay, performed in presence of Protein C Activator (Protac®, extracted from snake venom Agkistrodon Contortrix), phospholipids, contact phase activator, and calcium.
In the first step, the diluted assayed plasma is mixed with Protein C deficient plasma (R1). Then, the Activator Reagent (R2), in a constant and optimised concentration, is added. Clotting is initiated by the addition of Calcium (Ca2+). Clotting time is then recorded. Protein C being the limiting factor, there is a direct linear relationship, between the Protein C concentration and the corresponding clotting time.
This method will detect all mutations affecting Protein S and phospholipid interactions (type 2b) since those are only detected by clotting assays.

Ordering information

Product Name Assay principle Reference Package
HEMOCLOTTM  Protein C  (CE-IVD) in vitro clotting assay for the quantitative determination of Protein C (PC) in human citrated plasma, with a manual or automated method.  CK031K   3 x 20 tests

Additional Assays

Product Name Assay principle Reference Package
BIOPHENTM  Protein C   (CE-IVD) i Chromogenic method for in vitro quantitative determination of Protein C activity on human citrated plasma, using manual or automated method. Reagents are in the liquid presentation, ready to use (LRT, Liquid Reagent Technology). 221211  R1: 3 x 3 ml

R2: 3 x 3 ml