Pyrotell®-T lysate is used to quantify endotoxin in kinetic turbidimetric tests. Pyrotell-T lysate can be used with the Pyros Kinetix® tube reader and also incubating microplate readers. When used with the Pyros Kinetix tube reader, Pyrotell-T gives a highly economic, flexible and sensitive LAL assay.
It can be used for a wide variety of endotoxin tests, ranging from standard water testing to samples requiring high sensitivity, such as intrathecal products and those requiring high dilutions to overcome interference.
When used in a Pyros Kinetix tube reader, the maximum sensitivity is 0.001 EU/mL, which is 5 times more sensitive than any other LAL assays. The unique formulation of Pyrotell-T lysate allows a wide range of standard curves to be used, giving the user flexibility, speed, and ease in performing assays. When used in a microplate reader, the maximum sensitivity is 0.005 EU/mL.
Sample to Lysate Ratio
The ratio of sample: LAL is determined by personal preference and sample chemistry (interference patterns). A sample: LAL ratio for Pyrotell-T of 1:1 or 4:1 can be used according to user choice (and validation requirements).
The sample and lysate are added to test tubes or a microplate and incubated at 37°C. The time of incubation is dependent on the lowest standard concentration in the standard curve. Software is used to construct the standard curve and calculate the endotoxin concentrations.
Once reconstituted, Pyrotell-T lysate is stable for 24 hours, if stored at 2–8°C. Pyrotell-T lysate may be frozen once and will retain activity for as long as 3 months if stored at or below -20°C.
Pyrotell-T lysate is available in individual multi-test vials. Each vial contains reagent for approximately 96 tests.
|5 mL/vial 5 Pack (250 tests)|